Microbial Genomic Resource Repository

Microbial Genomic Resource Repository (MGRR) at National Bureau of Agriculturally Important Microorganism (NBAIM), Mau Nath Bhanjan, is a facility that preserves and conserves the genetic material of microorganisms, maintained in selected hosts or cloned and maintained in plasmids, accompanying the data details. This new organizational structure indicates the high importance and visibility that NBAIM places on our role as custodians of microorganisms and its related genetic resources. The policies and procedures represent evaluation, maintenance, regeneration, distribution and documentation of genetic resources at MGRR. MGRR maintains genetic materials like whole genome shotgun and cDNA/EST libraries, PAC/BAC/YAC clone vectors, component cells from sequencing projects, promoter DNA-fragments with reporter genes, RFLP probes specific for different microbes and expression vectors. Genetic material at MGRR can be deposited free of charge in the public collections and will be available for any third party under the terms of the material transfer agreement. MGRR provides a safe deposit to the genes and genetic elements with associated information for long-term stable preservation of microbial genetic resources. It is equipped with improved infrastructure and techniques for conserving diverse genetic materials.

The center is enriched with genomic DNA of different groups of fungi which include Myxomycetes, Mastigomycetes, Zygomycetes, Ascomycetes , Basidiomycetes and Deuteromycetes in addition to bacteria, actinomycetes and blue green algae (BGA).

# Genomic DNA:

A total of 1088 Genomic DNA from bacteria, 62 Genomic DNA from Actinomycetes and 568 Genomic DNA from fungal isolates of different species were extracted and have been kept in vials with concentration of 100ng per microliter and stored at -20 ⁰C.

  1. Genomic Library and Environmental Library:
  2. 6240 plasmid clones of shotgun Genomic Library of Mesorhizobium ciceri Ca181.
  3. 120 clones of 16S rRNA gene library from effluent irrigated soils.
  4. Plasmid and vectors:
  5. Plasmids: pDE1, pDE2, pDE3, pMYZ, ANEp4, ANEp6, pGEM10X-pYr4, pXACreII, pZHK2, poT-HY9, p444, pAL1, p474, pAL2 Lifac, pAL3 Lifact, ANEpZ, pSTA14, pB-142, Tn5 lzac2, DH5α, Tn5 LacZ1, pNCH3H4, ECO K12, Tn5 Kan, PBX17, PDP485, pHP2, PVG110, PLuc∆6B5, PDP6031, PBC-hygro, PMT-BFP, PHAN1, PC5N9, p35R2, pMG2254, pBT6, pR554, pJH19, pOT-Luc, pMYx10, pPND1, pSK700, pVG101.
  6. Vectors: PCCG:N-GFP, pMF332, PXD-RFP4, pGFP.hph:lexp, pMG1656, pMF331, pMG1648, pAC2-Lifact, AN1P p8, pN-3XHA. pA081, pM4X2, p500, pMT-3xHA

# 16S rRNA gene amplified products:

296 amplified 16S rRNA gene products used for identification of isolates from Rhizosphere, Psychrophile, Thermophiles and Actinomycetes which includes Rhizobium, Pseudomonas, Bacillus, Arthrobacter, Acinetobacter, Sphingomonas, Stenotrophomonas, Klebsiella, Bacillus tequilensis, Azotobactor, Burkholderia, Serretia and Agrobacterium, Lysinibacillus xylanilyticus, Enterobacter cloacae, Stenotrophomonas sp., Achromobacter sp., Microbacterium sp., Pseudomonas stutzeri, Microbacterium sp., Staphylococcus pasteuri, Streptomyces champavatii, Streptomyces violaceus, Streptomyces albus, Streptomyces sp, Streptomyces albidoflavus, Streptomyces albovinaceus, Streptomyces levis, Streptomyces purpurascens, Streptomyces coelicolor, Streptomyces sp., Streptomyces rochei, Streptomyces fungicidicus etc., has been amplified and stored at -20 *C.

# Amplified functional GENE products:

100 No. (GlnD, Gnd, gap, NodC, nodA, nodD, edD, rpoB, gacA, rpoD and prnD gene) of different structural as well as functional gene has been amplified, purified and submitted in MGRR.
MGRR has generated a well equipped world class laboratory with all modern equipments like Robotic DNA extractor, Pyrosequencer, Gel imaging system, Electroporator, High throughput gel electrophoresis system, Pulse-Field Gel Electrophoresis, Growth Kinetic Analizer and Automated growth Media Preparator etc.

To deposit the genetic material send the genetic material along with dully filled MTA and passport data submission form.